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Introduction of 3,5-Dinitrosalicylic acid.
3,5-Dinitrosalicylic acid (DNS) is an organic compound that is commonly used in biochemical research. It is a derivative of salicylic acid, where two nitro groups are attached to the benzene ring at the 3 and 5 positions. The chemical formula for DNS is C7H4N2O7, and it is known for its distinctive yellow to orange color.
DNS is primarily used as a reagent in the DNS assay, which is a colorimetric method for detecting reducing sugars and other reducing molecules. When DNS reacts with reducing sugars, it forms a reddish-brown compound that can be measured spectrophotometrically. This reaction is useful for quantifying the concentration of reducing sugars in various samples, such as in the analysis of enzymatic reactions involving carbohydrates.
Description of 3,5-Dinitrosalicylic acid from Wuhan Fortuna Chemical Co.,Ltd
Basic Information of 3,5-Dinitrosalicylic acid | |
Product Name | 3,5-Dinitrosalicylic acid |
CAS NO. | 609-99-4 |
Apperance | white crystalline powder |
Assay | 99% |
Pacakge | 1kg/Foil Bag,25kg/Drum/Box |
MOQ | 1kg |
Storage | Cool and dry place |
Shelf Life | 2 Years |
Brand | Fortunachem |
Specifications(COA) of High Purity 99% 3,5-Dinitrosalicylic acid CAS 609-99-4 Pharmaceutical intermediate
Items | Specifications | Results |
Appearance | white crystalline powder | Conforms |
Melting Point | 172-174（℃） | 172 |
Sulphated Ash | ≤0.1% | 0.05 |
Water content | ≤0.5（%） | 0.18 |
Solubility | Complete solution in water | Conforms |
Assay | 99.0% | 99.0% |
Conclusion | QUALIFIED. | |
Introduction of 3,5-Dinitrosalicylic acid.
3,5-Dinitrosalicylic acid (DNS) is an organic compound that is commonly used in biochemical research. It is a derivative of salicylic acid, where two nitro groups are attached to the benzene ring at the 3 and 5 positions. The chemical formula for DNS is C7H4N2O7, and it is known for its distinctive yellow to orange color.
DNS is primarily used as a reagent in the DNS assay, which is a colorimetric method for detecting reducing sugars and other reducing molecules. When DNS reacts with reducing sugars, it forms a reddish-brown compound that can be measured spectrophotometrically. This reaction is useful for quantifying the concentration of reducing sugars in various samples, such as in the analysis of enzymatic reactions involving carbohydrates.
The structure of 3,5-Dinitrosalicylic acid includes:
A benzene ring
A carboxyl group (-COOH) at the 1 position
A hydroxyl group (-OH) at the 2 position
Nitro groups (-NO2) at the 3 and 5 positions
This combination of functional groups makes DNS a versatile reagent in analytical chemistry, particularly in the field of carbohydrate research.
Application of 3,5-Dinitrosalicylic acid.
3,5-Dinitrosalicylic acid (DNS) is widely used in biochemical and analytical chemistry applications, particularly for its role in the DNS assay. Here are some of its primary applications:
1. Quantification of Reducing Sugars:
DNS Assay: The most common application of DNS is in the DNS assay, a colorimetric method used to quantify reducing sugars such as glucose, maltose, and lactose. When DNS reacts with reducing sugars, it forms a reddish-brown compound that can be measured spectrophotometrically, typically at a wavelength of around 540 nm. This assay is widely used in various fields, including food science, fermentation technology, and enzymology.
2. Enzyme Activity Measurement:
Enzymatic Reactions: DNS is used to measure the activity of enzymes that produce reducing sugars as products. For example, it is used to monitor the activity of amylases, cellulases, and other carbohydrate-hydrolyzing enzymes. By quantifying the amount of reducing sugar produced, researchers can determine the enzyme’s activity and efficiency.
3. Biotechnology and Industrial Applications:
Fermentation Processes: In industrial biotechnology, the DNS assay is used to monitor the progress of fermentation processes by measuring the concentration of reducing sugars in the fermentation broth. This helps in optimizing the fermentation conditions and improving yield.
Biofuel Production: In biofuel research, DNS is used to measure the breakdown of biomass into fermentable sugars, which are then converted into biofuels like ethanol.
4. Food and Beverage Industry:
Quality Control: The DNS assay is employed in the food and beverage industry to assess the sugar content in various products, ensuring quality control and compliance with nutritional labeling requirements.
5. Research and Development:
Carbohydrate Research: DNS is used in research laboratories to study carbohydrate metabolism, the kinetics of enzymatic reactions involving carbohydrates, and the structural properties of polysaccharides.
6. Educational Purposes:
Teaching Laboratories: The DNS assay is often included in biochemistry and microbiology laboratory courses to teach students about enzyme kinetics, carbohydrate chemistry, and spectrophotometric analysis.
7. Medical and Clinical Research:
Diabetes Research: Although less common, DNS can be used in research settings to study glucose levels and carbohydrate metabolism in the context of diabetes research.
Overall, 3,5-Dinitrosalicylic acid is a versatile reagent with a wide range of applications in scientific research, industrial processes, and educational settings, primarily due to its ability to detect and quantify reducing sugars.
FAQ From Wuhan Fortuna Chemical Co.,Ltd | |
Q | Can I get a sample for free ? |
A | For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test. |
Q | How to confirm the product quality before placing orders ? |
A | Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order. |
Q | What is the payment you accept? |
A | For small and low value order, we accept CreditCard, MaterCard,VISA,Western Union and T/T.For large order, we can do T/T and L/C. Any pay on delivery or pay-after are not available for us. |
Q | What is your shipment way ? |
A | For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement. |
Q | How long is your after-sales service? |
A | We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company. |
Q | How do you treat quality complaint ? |
A | We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers. |
Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is the members of Hubei E-commerce Chamber.
Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.
We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.
Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.
Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide 3,5-Dinitrosalicylic acid with good quality and best price, welcome to contact us to get COA/TDS/MSDS of 3,5-Dinitrosalicylic acid.
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