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Introduction of 4-Nitrophenyl β-D-Cellobioside.
4-Nitrophenyl β-D-Cellobioside is a synthetic substrate commonly used in biochemical assays to study the activity of cellulase enzymes. It is a derivative of cellobiose, which is a disaccharide composed of two glucose molecules linked by a β(1→4) glycosidic bond. In this compound, the reducing end of the cellobiose is modified with a 4-nitrophenyl group.
When cellulase enzymes act on 4-Nitrophenyl β-D-Cellobioside, they cleave the glycosidic bond, releasing 4-nitrophenol. The release of 4-nitrophenol can be easily monitored because it has a distinct yellow color and absorbs light at a wavelength of 405 nm. This property makes 4-Nitrophenyl β-D-Cellobioside a useful tool for measuring cellulase activity in various research and industrial applications.
In summary, 4-Nitrophenyl β-D-Cellobioside is a valuable substrate for studying cellulase enzymes, providing a convenient and quantifiable way to assess enzyme activity through the release of 4-nitrophenol.
Description of 4-Nitrophenyl β-D-Cellobioside from Wuhan Fortuna Chemical Co.,Ltd
Basic Information of 4-Nitrophenyl β-D-Cellobioside | |
Product Name | 4-Nitrophenyl β-D-Cellobioside |
CAS NO. | 3482-57-3 |
Apperance | White Crystalline Powder |
Assay | 98.2% |
Pacakge | 1kg/Foil Bag,25kg/Drum/Box |
MOQ | 1kg |
Storage | Cool and dry place |
Shelf Life | 2 Years |
Brand | Fortunachem |
Specifications(COA) of High Quality 98.2% 4-Nitrophenyl β-D-Cellobioside CAS NO 3482-57-3 Biochemical in Stock
Items | Specifications | Results |
Appearance | White/Pale Yellow Crystalline Powder | White Crystalline Powder |
Solubility, 1 % Water | Clear, Colorless Solution | Clear Solution |
TLC（acid ester/methyl alcohol=6/1） | Single Spot | Single Spot |
Melting Point | 250℃ | 250.1-250.6℃ |
Water content | Max 2% | 0.7% |
[a](c=0.5,in H2O) | -80° ～ -84° | -82.9° |
HPLC | 98% | 98.2% |
Conclusion | The product conforms to the standard of CP2010. | |
Introduction of 4-Nitrophenyl β-D-Cellobioside.
4-Nitrophenyl β-D-Cellobioside is a synthetic substrate commonly used in biochemical assays to study the activity of cellulase enzymes. It is a derivative of cellobiose, which is a disaccharide composed of two glucose molecules linked by a β(1→4) glycosidic bond. In this compound, the reducing end of the cellobiose is modified with a 4-nitrophenyl group.
When cellulase enzymes act on 4-Nitrophenyl β-D-Cellobioside, they cleave the glycosidic bond, releasing 4-nitrophenol. The release of 4-nitrophenol can be easily monitored because it has a distinct yellow color and absorbs light at a wavelength of 405 nm. This property makes 4-Nitrophenyl β-D-Cellobioside a useful tool for measuring cellulase activity in various research and industrial applications.
In summary, 4-Nitrophenyl β-D-Cellobioside is a valuable substrate for studying cellulase enzymes, providing a convenient and quantifiable way to assess enzyme activity through the release of 4-nitrophenol.
Application of 4-Nitrophenyl β-D-Cellobioside.
4-Nitrophenyl β-D-Cellobioside is primarily used in biochemical and enzymatic assays to study the activity of cellulase enzymes. Here are some specific applications:
Enzyme Activity Assays: It is used to measure the activity of cellulase enzymes. When cellulases cleave the glycosidic bond in 4-Nitrophenyl β-D-Cellobioside, they release 4-nitrophenol, which can be quantitatively measured by its absorbance at 405 nm. This allows researchers to determine the rate of enzyme activity.
Enzyme Kinetics: Researchers use this substrate to study the kinetics of cellulase enzymes, including parameters like Km (Michaelis constant) and Vmax (maximum velocity). This helps in understanding the efficiency and mechanism of the enzyme.
Screening for Enzyme Inhibitors: It can be used in high-throughput screening assays to identify potential inhibitors of cellulase enzymes. By observing changes in the release of 4-nitrophenol, researchers can determine the effectiveness of various compounds in inhibiting cellulase activity.
Characterization of Mutant Enzymes: In genetic and protein engineering studies, 4-Nitrophenyl β-D-Cellobioside can be used to characterize the activity of mutant cellulase enzymes. This helps in understanding how specific mutations affect enzyme function.
Industrial Applications: In industries such as biofuel production, where cellulases are used to break down cellulose into fermentable sugars, 4-Nitrophenyl β-D-Cellobioside can be used to monitor and optimize enzyme performance.
Environmental Studies: It can be used to study the degradation of cellulose in various environmental samples, helping to understand the role of cellulases in natural ecosystems.
Quality Control: In the production of cellulase enzymes, 4-Nitrophenyl β-D-Cellobioside can be used for quality control to ensure that the enzyme preparations have the desired activity.
Overall, 4-Nitrophenyl β-D-Cellobioside is a versatile and valuable tool in both research and industrial settings for studying and utilizing cellulase enzymes.
FAQ From Wuhan Fortuna Chemical Co.,Ltd | |
Q | Can I get a sample for free ? |
A | For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test. |
Q | How to confirm the product quality before placing orders ? |
A | Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order. |
Q | What is the payment you accept? |
A | For small and low value order, we accept CreditCard, MaterCard,VISA,Western Union and T/T.For large order, we can do T/T and L/C. Any pay on delivery or pay-after are not available for us. |
Q | What is your shipment way ? |
A | For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement. |
Q | How long is your after-sales service? |
A | We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company. |
Q | How do you treat quality complaint ? |
A | We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers. |
Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is the members of Hubei E-commerce Chamber.
Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.
We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.
Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.
Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide 4-Nitrophenyl β-D-Cellobioside with good quality and best price, welcome to contact us to get COA/TDS/MSDS of 4-Nitrophenyl β-D-Cellobioside.
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