Nitrazine Yellow CAS 5423-07-4 Wholesale & Bulk

Nitrazine Yellow, also known as Nitrazine Yellow indicator, is a synthetic dye and a pH-sensitive compound commonly used in various fields due to its ability to exhibit distinct color changes across a specific pH range. Chemically, it is a type of azo dye. Its primary application is as a pH indicator.

Key Properties:

  1. Chemical Composition:

    • It is classified as an azo compound, characterized by the presence of an -N=N- functional group.

    • Soluble in water and other polar solvents.


Nitrazine Yellow, also known as Nitrazine Yellow Indicator, is a pH-sensitive dye widely used in various applications. Its primary purpose is as a pH indicator due to its ability to change color in response to changes in pH. Here are the main applications:

1. Medical Diagnostics

  • Amniotic Fluid Leakage Detection: Nitrazine Yellow is used in obstetrics to detect premature rupture of membranes (PROM) in pregnancy. The dye is applied to vaginal fluid samples, and its color change indicates the presence of amniotic fluid, which has a higher pH (7.0-7.5) compared to normal vaginal secretions (pH 4.5-6.0).

  • Urinary Tract and Vaginal pH Testing: Used to assess infections or imbalances, as many conditions are associated with changes in pH.

2. Laboratory Use

  • Microbiology and Biochemistry: Used in culture media and biochemical experiments where monitoring pH changes is necessary.

  • Titration: Helps visually determine the endpoint of titrations in analytical chemistry when the pH is in its active range.

3. Educational Use

  • Commonly used in teaching laboratories to demonstrate pH changes and acid-base reactions due to its clear color transitions.

4. Industrial Applications

  • Utilized in industrial processes to monitor and control pH in solutions where precise pH management is essential.

Color Changes of Nitrazine Yellow:

  • pH < 6.0: Yellow

  • pH > 7.0: Blue

This makes it particularly useful for applications where distinguishing neutral to slightly basic conditions is important.


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