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Acid Blue 83, also known as Coomassie Brilliant Blue R-250, is a synthetic dye widely used in various applications, including scientific, industrial, and artistic fields. Here's an overview:
Type: Acid dye (anionic dye).
Chemical Formula: C45H44N3NaO7S2.
Molecular Weight: Approximately 825.97 g/mol.
Appearance: A dark blue powder.
Acid Blue 83, also known as Coomassie Brilliant Blue R-250, has several applications across different fields due to its chemical properties. Below are its primary uses:
Protein Staining in Electrophoresis:
Used extensively in SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) to stain proteins.
Helps in visualizing protein bands by binding to protein molecules through ionic and hydrophobic interactions.
Bradford Protein Assay:
Utilized to determine protein concentration. Acid Blue 83 changes color when it binds to proteins, with absorbance measured at 595 nm.
Western Blot Analysis:
Used for staining proteins transferred to membranes for identification and quantification.
Dyeing wool, silk, and nylon fabrics due to its strong affinity for protein-based fibers.
Imparts a deep blue color, known for its vibrancy and resistance to fading.
Used as a coloring agent in the manufacturing of certain types of inks.
Employed to tint or dye paper products.
Occasionally used as a colorant in artistic mediums.
Can be included in specific cosmetic formulations (subject to safety regulations).
Common in teaching labs for demonstrating protein separation and quantification techniques.
Serves as a visually engaging tool for learning about electrophoresis and staining methods.
Used in research to detect and study protein contaminants in environmental samples.
Can assist in forensic applications involving protein residue analysis.
High Sensitivity: Effective in detecting proteins down to nanogram levels.
Cost-Effective: Widely available and relatively inexpensive.
Ease of Use: Readily soluble and compatible with many biochemical protocols.
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