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5-Bromo-4-chloro-3-indolyl-beta-D-galactoside, commonly abbreviated as X-Gal, is a chromogenic substrate used primarily in molecular biology to detect the activity of the enzyme beta-galactosidase.
Description of X-GAL from Wuhan Fortuna Chemical Co.,Ltd
Basic Information of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside; X-GAL | |
Product Name | X-GAL |
CAS NO. | 7240-90-6 |
Apperance | White powder |
Assay | 98% |
Package | 1g/bag |
Brand | Fortunachem |
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside, commonly abbreviated as X-Gal, is a chromogenic substrate used primarily in molecular biology to detect the activity of the enzyme beta-galactosidase.
Chemical Structure: X-Gal is an indole derivative, chemically modified with bromine and chlorine substituents.
Appearance: It is a white to light yellow powder in its dry form.
Solubility: Typically dissolved in dimethylformamide (DMF) or dimethyl sulfoxide (DMSO) for use in experiments.
In the presence of beta-galactosidase, X-Gal is cleaved into galactose and a blue indole derivative, which precipitates out as an intense blue color. This color change is visible, allowing for easy identification of cells or colonies expressing the enzyme.
Blue-White Screening in Cloning: X-Gal is extensively used in conjunction with the lacZ gene in bacterial cloning. When introduced into an E. coli strain with a lacZ mutation (such as lacZΔM15), colonies expressing functional beta-galactosidase will cleave X-Gal and turn blue. This makes it easy to distinguish recombinant (white) from non-recombinant (blue) colonies in plasmid cloning.
Reporter Gene Assays: The lacZ gene, which encodes beta-galactosidase, is sometimes used as a reporter gene in genetic studies. X-Gal is used to detect the expression of lacZ, providing a visual indicator of gene expression in cells or tissues.
Histochemical Staining: In histology, X-Gal staining can localize beta-galactosidase activity within tissues. This application is often used in animal models where lacZ is inserted as a reporter gene, allowing researchers to visualize gene expression patterns within an organism.
Simple and Visual: The intense blue color allows for easy detection without advanced equipment.
Non-toxic: X-Gal is generally non-toxic to bacteria and cells at typical experimental concentrations.
Sensitivity to Light and Temperature: X-Gal solutions are sensitive to light and should be stored away from direct light to prevent degradation.
In summary, X-Gal is an essential tool in molecular biology, particularly in genetic engineering, gene expression studies, and histological applications, due to its ability to produce a visible color change in the presence of beta-galactosidase.
FAQ From Wuhan Fortuna Chemical Co.,Ltd | |
Q | Can I get a sample for free ? |
A | For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test. |
Q | How to confirm the product quality before placing orders ? |
A | Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order. |
Q | What is the payment you accept? |
A | For small and low value order, we accept CreditCard, MaterCard,VISA,Western Union and T/T.For large order, we can do T/T and L/C. Any pay on delivery or pay-after are not available for us. |
Q | What is your shipment way ? |
A | For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement. |
Q | How long is your after-sales service? |
A | We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company. |
Q | How do you treat quality complaint ? |
A | We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers. |
Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is the members of Hubei E-commerce Chamber.
Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.
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Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.
Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide X-GAL with good quality and best price, welcome to contact us to get COA/TDS/MSDS of X-GAL
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